Project

Part:BBa_K404123:Design

Designed by: Freiburg Bioware 2010   Group: iGEM10_Freiburg_Bioware   (2010-10-11)

[AAV2]-left-ITR_phTERT_betaglobin_mVenus_hGH_[AAV2]-right-ITR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1122
    Illegal AgeI site found at 1842
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2244


Design Notes

All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard. Fusion of two parts was conducted following the proposed BioBrick Assembly Strategy (https://parts.igem.org/Assembly:Standard_assembly). Since the gene of interest was designed in the RFC25 standard, BioBrick assembly leads to a scar and the additional restriction enzymes NgoMIV and AgeI.

Source

pAAV_MCS provided by Stratagene

References